Observation of living cells follows below procedures.
Negative Staining
Microorganism will not stain in this method, but the medium/environment will stain. Staining will kill the microbe hence negative staining provides us the facility to observe the live cells. This method will be discussed more under the topic practical.
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Medium is stained but
not cells
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Hanging Drop Procedure
In this technique, a drop of medium containing cells is allowed to hang in the cavity slide. This method is used to study the motility of microorganism. There are two movements that can be observed, true movement and Brownian movement. Microorganism changes its position in a true movement whereas in Brownian movement the organism oscillates at its place and does not change the position in the field. Brownian movement can be explained based on the bombardment of small particles suspended in the fluid, with the cell. This method will be discussed more under the topic practical.
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