Streak plate or spread plate
This will be discussed under the section “practicals”.
Dilution plating
A series of test tubes containing 9 ml of sterile distilled water or 0.85% NaCl, peptone water and Ringer's solution has to be prepared which are called dilution blanks.1ml of the initial or original sample is transferred into the 1st test tube (10-1).Shake.Next 1ml of 10-1 solution is transferred into the next test tube (10-2). This procedure is carried out for another 5/6 test tubes. A sterile pipette is used to transfer solutions at each step. Then a pour plate is made for each and every dilution. (will be discussed at practicals section).
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Dilution plating
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Dilution culture
As mentioned in dilution plating a dilution series is prepared. Cloudiness/turbidity is measured in each test tube using a spectrophotometer, turbidimeter or nephelometer. In this method, cloudiness or turbidity of the medium is an indication of the microbial growth. We assume that the last dilution where turbidity observed is from a single cell which is pure culture.
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